Journal: Infection and Immunity

Article Title: Mannoprotein Cig1 contributes to the immunogenicity of a heat-killed F-box protein Fbp1 Cryptococcus neoformans vaccine model

doi: 10.1128/iai.00355-25

Figure Lengend Snippet: Capsule is required for full HK - fbp1 vaccine-mediated protection. ( A ) Schematic of the vaccination strategy. Mice are vaccinated with HK- fbp1 or acapsular strains (HK- cap59 or HK- cap59 fbp1 ) 42 days and 12 days before challenge with 1 × 10 4 H99 wild-type strain. ( B–D ) Day 7 post-H99 challenge immune response. IFN-γ producing CD4 + T cells in BALF ( B ), IFN-γ cytokine readout from isolated T-cells of vaccinated mice presented H99 antigen ( C ), qPCR from total lung tissue and ratio of CD4 + to CD8 + T cells in the vaccinated cohorts ( D ). * P < 0.05, ** P < 0.001, and **** P < 0.0001, Kruskal-Wallis test with Dunn’s multiple comparison test. ( E–F ) Survival curve of mice vaccinated with HK- fbp1 or acapsular strains ( E ) and fungal burden ( F ) of remaining survivors. * P < 0.05; **** P < 0.0001 (log rank [Mantel-Cox] test). Data are representative of two independent experiments.

Article Snippet: Expressions of CIG1, CMP1, CFO1, MP88, MP84, MP98, MP115, and GAPDH were analyzed using SYBRGREEN advantage QPCR premix reagents (Takara).

Techniques: Isolation, Comparison